cell culture tf 1 cells Search Results


tf1 cell line  (European Collection of Authenticated Cell Cultures)
90
European Collection of Authenticated Cell Cultures tf1 cell line
(A) Schematic diagrams of conventional and chimeric scFv specific for CD123. CARs 1 to 4: CD123-specific CARs generated by fusing V L and V H chains of mAbs specific to CD123. CARs 5–9: Chimeric scFvs created by mix-and-matching V L and V H chains. The scFvs of CARs 1–9 were fused to the signaling domains of CD28 and CD3ζ via CD8α hinge and TM domains. CAR-10 was derived by fusing the chimeric scFv from CAR 6 to the CD3ζ and CD28 endo-domains via the IgG4 hinge and CD28 TM domains. (B) Expansion kinetics of CARs 1–4 (left) and CARs 5–10 (right) over a period of 28 days from day 1 following electroporation of SB CAR plasmids. Data are pooled from 3 donors; graph displays mean ± SEM (C) CAR expression on Day 21 after electroporation. CAR expression was detected by CD123 recombinant protein fused to Fc followed by serial staining with fluorescence-labeled anti-Fc and anti-CD3 antibodies. (D) in vitro lysis of CD123 + target cells Nalm 6, <t>TF1,</t> 293T-parental cells, CD123-transfected 293T cells, and 123 neg by CAR + T cells. Histograms represent the mean ± SEM, n = 3. (E) CAR + T cell killing of BM-derived target cells. Mononuclear cells were isolated from normal human bone marrow samples and sorted for expression of lineage markers into lineage-positive (Lin + ) and lineage-negative (Lin neg ) groups. The latter presumable reflects the HSC pool. The BM-derived cells were then labeled with PKH-26 and incubated with CAR + T cells for 2 days before vitality was assessed by flow cytometry. The percent lysis compared to controls is shown. Histograms represent the mean ± SEM of 3 replicates. (F) Interferon-γ release by CAR + T cells after exposure to CD123. Day 28 CD123-specific CAR + T cells were incubated for 24 hours with Nalm-6 cells (CD123 + ), 293T cells (CD123 neg ), or alone, then the supernatant tested for cytokine expression using Biolegend plex Th1 cytokine capture beads, measured by flow cytometry. Results for IFN-γ are shown; other cytokines were not detectible over background. Histograms represent mean ± SEM for 2 replicates from 2 different experiments.
Tf1 Cell Line, supplied by European Collection of Authenticated Cell Cultures, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tf1 cell line/product/European Collection of Authenticated Cell Cultures
Average 90 stars, based on 1 article reviews
tf1 cell line - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
erythroleukemia cell line tf-1  (European Collection of Authenticated Cell Cultures)
90
European Collection of Authenticated Cell Cultures erythroleukemia cell line tf-1
(A) Schematic diagrams of conventional and chimeric scFv specific for CD123. CARs 1 to 4: CD123-specific CARs generated by fusing V L and V H chains of mAbs specific to CD123. CARs 5–9: Chimeric scFvs created by mix-and-matching V L and V H chains. The scFvs of CARs 1–9 were fused to the signaling domains of CD28 and CD3ζ via CD8α hinge and TM domains. CAR-10 was derived by fusing the chimeric scFv from CAR 6 to the CD3ζ and CD28 endo-domains via the IgG4 hinge and CD28 TM domains. (B) Expansion kinetics of CARs 1–4 (left) and CARs 5–10 (right) over a period of 28 days from day 1 following electroporation of SB CAR plasmids. Data are pooled from 3 donors; graph displays mean ± SEM (C) CAR expression on Day 21 after electroporation. CAR expression was detected by CD123 recombinant protein fused to Fc followed by serial staining with fluorescence-labeled anti-Fc and anti-CD3 antibodies. (D) in vitro lysis of CD123 + target cells Nalm 6, <t>TF1,</t> 293T-parental cells, CD123-transfected 293T cells, and 123 neg by CAR + T cells. Histograms represent the mean ± SEM, n = 3. (E) CAR + T cell killing of BM-derived target cells. Mononuclear cells were isolated from normal human bone marrow samples and sorted for expression of lineage markers into lineage-positive (Lin + ) and lineage-negative (Lin neg ) groups. The latter presumable reflects the HSC pool. The BM-derived cells were then labeled with PKH-26 and incubated with CAR + T cells for 2 days before vitality was assessed by flow cytometry. The percent lysis compared to controls is shown. Histograms represent the mean ± SEM of 3 replicates. (F) Interferon-γ release by CAR + T cells after exposure to CD123. Day 28 CD123-specific CAR + T cells were incubated for 24 hours with Nalm-6 cells (CD123 + ), 293T cells (CD123 neg ), or alone, then the supernatant tested for cytokine expression using Biolegend plex Th1 cytokine capture beads, measured by flow cytometry. Results for IFN-γ are shown; other cytokines were not detectible over background. Histograms represent mean ± SEM for 2 replicates from 2 different experiments.
Erythroleukemia Cell Line Tf 1, supplied by European Collection of Authenticated Cell Cultures, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/erythroleukemia cell line tf-1/product/European Collection of Authenticated Cell Cultures
Average 90 stars, based on 1 article reviews
erythroleukemia cell line tf-1 - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
il-6–responsive human tf-1 cells  (European Collection of Authenticated Cell Cultures)
90
European Collection of Authenticated Cell Cultures il-6–responsive human tf-1 cells
(A) Schematic diagrams of conventional and chimeric scFv specific for CD123. CARs 1 to 4: CD123-specific CARs generated by fusing V L and V H chains of mAbs specific to CD123. CARs 5–9: Chimeric scFvs created by mix-and-matching V L and V H chains. The scFvs of CARs 1–9 were fused to the signaling domains of CD28 and CD3ζ via CD8α hinge and TM domains. CAR-10 was derived by fusing the chimeric scFv from CAR 6 to the CD3ζ and CD28 endo-domains via the IgG4 hinge and CD28 TM domains. (B) Expansion kinetics of CARs 1–4 (left) and CARs 5–10 (right) over a period of 28 days from day 1 following electroporation of SB CAR plasmids. Data are pooled from 3 donors; graph displays mean ± SEM (C) CAR expression on Day 21 after electroporation. CAR expression was detected by CD123 recombinant protein fused to Fc followed by serial staining with fluorescence-labeled anti-Fc and anti-CD3 antibodies. (D) in vitro lysis of CD123 + target cells Nalm 6, <t>TF1,</t> 293T-parental cells, CD123-transfected 293T cells, and 123 neg by CAR + T cells. Histograms represent the mean ± SEM, n = 3. (E) CAR + T cell killing of BM-derived target cells. Mononuclear cells were isolated from normal human bone marrow samples and sorted for expression of lineage markers into lineage-positive (Lin + ) and lineage-negative (Lin neg ) groups. The latter presumable reflects the HSC pool. The BM-derived cells were then labeled with PKH-26 and incubated with CAR + T cells for 2 days before vitality was assessed by flow cytometry. The percent lysis compared to controls is shown. Histograms represent the mean ± SEM of 3 replicates. (F) Interferon-γ release by CAR + T cells after exposure to CD123. Day 28 CD123-specific CAR + T cells were incubated for 24 hours with Nalm-6 cells (CD123 + ), 293T cells (CD123 neg ), or alone, then the supernatant tested for cytokine expression using Biolegend plex Th1 cytokine capture beads, measured by flow cytometry. Results for IFN-γ are shown; other cytokines were not detectible over background. Histograms represent mean ± SEM for 2 replicates from 2 different experiments.
Il 6–Responsive Human Tf 1 Cells, supplied by European Collection of Authenticated Cell Cultures, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/il-6–responsive human tf-1 cells/product/European Collection of Authenticated Cell Cultures
Average 90 stars, based on 1 article reviews
il-6–responsive human tf-1 cells - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier

Image Search Results


(A) Schematic diagrams of conventional and chimeric scFv specific for CD123. CARs 1 to 4: CD123-specific CARs generated by fusing V L and V H chains of mAbs specific to CD123. CARs 5–9: Chimeric scFvs created by mix-and-matching V L and V H chains. The scFvs of CARs 1–9 were fused to the signaling domains of CD28 and CD3ζ via CD8α hinge and TM domains. CAR-10 was derived by fusing the chimeric scFv from CAR 6 to the CD3ζ and CD28 endo-domains via the IgG4 hinge and CD28 TM domains. (B) Expansion kinetics of CARs 1–4 (left) and CARs 5–10 (right) over a period of 28 days from day 1 following electroporation of SB CAR plasmids. Data are pooled from 3 donors; graph displays mean ± SEM (C) CAR expression on Day 21 after electroporation. CAR expression was detected by CD123 recombinant protein fused to Fc followed by serial staining with fluorescence-labeled anti-Fc and anti-CD3 antibodies. (D) in vitro lysis of CD123 + target cells Nalm 6, TF1, 293T-parental cells, CD123-transfected 293T cells, and 123 neg by CAR + T cells. Histograms represent the mean ± SEM, n = 3. (E) CAR + T cell killing of BM-derived target cells. Mononuclear cells were isolated from normal human bone marrow samples and sorted for expression of lineage markers into lineage-positive (Lin + ) and lineage-negative (Lin neg ) groups. The latter presumable reflects the HSC pool. The BM-derived cells were then labeled with PKH-26 and incubated with CAR + T cells for 2 days before vitality was assessed by flow cytometry. The percent lysis compared to controls is shown. Histograms represent the mean ± SEM of 3 replicates. (F) Interferon-γ release by CAR + T cells after exposure to CD123. Day 28 CD123-specific CAR + T cells were incubated for 24 hours with Nalm-6 cells (CD123 + ), 293T cells (CD123 neg ), or alone, then the supernatant tested for cytokine expression using Biolegend plex Th1 cytokine capture beads, measured by flow cytometry. Results for IFN-γ are shown; other cytokines were not detectible over background. Histograms represent mean ± SEM for 2 replicates from 2 different experiments.

Journal: PLoS ONE

Article Title: Redirecting Specificity of T cells Using the Sleeping Beauty System to Express Chimeric Antigen Receptors by Mix-and-Matching of V L and V H Domains Targeting CD123 + Tumors

doi: 10.1371/journal.pone.0159477

Figure Lengend Snippet: (A) Schematic diagrams of conventional and chimeric scFv specific for CD123. CARs 1 to 4: CD123-specific CARs generated by fusing V L and V H chains of mAbs specific to CD123. CARs 5–9: Chimeric scFvs created by mix-and-matching V L and V H chains. The scFvs of CARs 1–9 were fused to the signaling domains of CD28 and CD3ζ via CD8α hinge and TM domains. CAR-10 was derived by fusing the chimeric scFv from CAR 6 to the CD3ζ and CD28 endo-domains via the IgG4 hinge and CD28 TM domains. (B) Expansion kinetics of CARs 1–4 (left) and CARs 5–10 (right) over a period of 28 days from day 1 following electroporation of SB CAR plasmids. Data are pooled from 3 donors; graph displays mean ± SEM (C) CAR expression on Day 21 after electroporation. CAR expression was detected by CD123 recombinant protein fused to Fc followed by serial staining with fluorescence-labeled anti-Fc and anti-CD3 antibodies. (D) in vitro lysis of CD123 + target cells Nalm 6, TF1, 293T-parental cells, CD123-transfected 293T cells, and 123 neg by CAR + T cells. Histograms represent the mean ± SEM, n = 3. (E) CAR + T cell killing of BM-derived target cells. Mononuclear cells were isolated from normal human bone marrow samples and sorted for expression of lineage markers into lineage-positive (Lin + ) and lineage-negative (Lin neg ) groups. The latter presumable reflects the HSC pool. The BM-derived cells were then labeled with PKH-26 and incubated with CAR + T cells for 2 days before vitality was assessed by flow cytometry. The percent lysis compared to controls is shown. Histograms represent the mean ± SEM of 3 replicates. (F) Interferon-γ release by CAR + T cells after exposure to CD123. Day 28 CD123-specific CAR + T cells were incubated for 24 hours with Nalm-6 cells (CD123 + ), 293T cells (CD123 neg ), or alone, then the supernatant tested for cytokine expression using Biolegend plex Th1 cytokine capture beads, measured by flow cytometry. Results for IFN-γ are shown; other cytokines were not detectible over background. Histograms represent mean ± SEM for 2 replicates from 2 different experiments.

Article Snippet: The TF1 cell line was obtained from the European Collection of Authenticated Cell Cultures (ECACC).

Techniques: Generated, Derivative Assay, Electroporation, Expressing, Recombinant, Staining, Fluorescence, Labeling, In Vitro, Lysis, Transfection, Isolation, Incubation, Flow Cytometry

(A) Overlay histograms display the flow cytometric analysis of CD123 expression on AML cell lines MV4-11, Molm-13, TF1, OCI-AML3, EL4-Parental and EL4-CD123. Isotype control is shown in grey, and specific staining by the unfilled black line. (B) Specific lysis of CD123-CD28 and CD123-CD137 CAR + T cells against AML cell lines EL4, CD123 neg OCI-Ly19, MV4-11, TF1, EL4-CD123, Molm-13, and OCI-AML3 assessed with a 4 hour chromium release assay. Histograms represent mean ± SEM, n = 3 (C) Flow cytometric analysis of CD123 expression on primary AML samples used in the co-culture assay depicted in (D) . Lysis of PKH-26 labeled primary AML cells by CD123-CD28 or CD123-CD137 CAR T cells at 1:1 ratio for 72 hours. CD19-specific CAR + T cells were used as a negative control.

Journal: PLoS ONE

Article Title: Redirecting Specificity of T cells Using the Sleeping Beauty System to Express Chimeric Antigen Receptors by Mix-and-Matching of V L and V H Domains Targeting CD123 + Tumors

doi: 10.1371/journal.pone.0159477

Figure Lengend Snippet: (A) Overlay histograms display the flow cytometric analysis of CD123 expression on AML cell lines MV4-11, Molm-13, TF1, OCI-AML3, EL4-Parental and EL4-CD123. Isotype control is shown in grey, and specific staining by the unfilled black line. (B) Specific lysis of CD123-CD28 and CD123-CD137 CAR + T cells against AML cell lines EL4, CD123 neg OCI-Ly19, MV4-11, TF1, EL4-CD123, Molm-13, and OCI-AML3 assessed with a 4 hour chromium release assay. Histograms represent mean ± SEM, n = 3 (C) Flow cytometric analysis of CD123 expression on primary AML samples used in the co-culture assay depicted in (D) . Lysis of PKH-26 labeled primary AML cells by CD123-CD28 or CD123-CD137 CAR T cells at 1:1 ratio for 72 hours. CD19-specific CAR + T cells were used as a negative control.

Article Snippet: The TF1 cell line was obtained from the European Collection of Authenticated Cell Cultures (ECACC).

Techniques: Expressing, Control, Staining, Lysis, Release Assay, Co-culture Assay, Labeling, Negative Control

(A) Schematic of the TF1 xenograft model. 2.5 × 10 6 TF1- effLuc -mKate cells were injected intravenously into NSG mice on day 0. On Day 5, tumor engraftment was quantified using non-invasive bioluminescence imaging (BLI), and mice were randomly divided into 3 groups: untreated (control), CD123-CD28-treated, or CD123-CD137-treated. CAR-treated mice were given infusions of T cells followed by IL-2 treatment and BLI on day 5, 11 and 20. Untreated mice received no T cells. (B) BLI images of mice display an overlay of luciferase activity, using the color scale shown on the right, displayed over the white-light image of the mice. (C) Histograms represent the luciferase activity measured by BLI for each group (** p < 0.01). (D) Kaplan-Meier curves display the survival analysis of xenograft mice treated with CD123-specific CAR T cells (** p < 0.01).

Journal: PLoS ONE

Article Title: Redirecting Specificity of T cells Using the Sleeping Beauty System to Express Chimeric Antigen Receptors by Mix-and-Matching of V L and V H Domains Targeting CD123 + Tumors

doi: 10.1371/journal.pone.0159477

Figure Lengend Snippet: (A) Schematic of the TF1 xenograft model. 2.5 × 10 6 TF1- effLuc -mKate cells were injected intravenously into NSG mice on day 0. On Day 5, tumor engraftment was quantified using non-invasive bioluminescence imaging (BLI), and mice were randomly divided into 3 groups: untreated (control), CD123-CD28-treated, or CD123-CD137-treated. CAR-treated mice were given infusions of T cells followed by IL-2 treatment and BLI on day 5, 11 and 20. Untreated mice received no T cells. (B) BLI images of mice display an overlay of luciferase activity, using the color scale shown on the right, displayed over the white-light image of the mice. (C) Histograms represent the luciferase activity measured by BLI for each group (** p < 0.01). (D) Kaplan-Meier curves display the survival analysis of xenograft mice treated with CD123-specific CAR T cells (** p < 0.01).

Article Snippet: The TF1 cell line was obtained from the European Collection of Authenticated Cell Cultures (ECACC).

Techniques: Injection, Imaging, Control, Luciferase, Activity Assay